Human stem cell technology and biology: a research guide and laboratory manual (Hoboken, 2011). - ОГЛАВЛЕНИЕ / CONTENTS
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ОбложкаHuman stem cell technology and biology: a research guide and laboratory manual / ed. by G.S.Stein et al. - Hoboken: Wiley-Blackwell, 2011. - xvii, 419 p.: ill. + CD-ROM. - Incl. bibl. ref. - Ind.: p.413-419. - ISBN 978-0-470-59545-9
 

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Оглавление / Contents
 
Foreword ....................................................... ix
Preface ........................................................ xi
Acknowledgments .............................................. xiii
Editors and Contributors ....................................... xv

SECTION I  INTRODUCTION
1  Introduction to Pluripotent Stem Cells: Biology and
   Applications ................................................. 3
   Maria Borowski and Gary S. Stein
2  Researching and Obtaining Established Stem Cell Lines ........ 9
   Mai X. Luong, Kelly P. Smith, and Gary S. Stein

SECTION II  LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE:
PLURIPOTENT STEM CELL CULTURE
3  Basics of Cell Culture ...................................... 19
   Alicia Allaire, Mai X. Luong, and Kelly P. Smith
4  The Stem Cell Laboratory .................................... 33
   Alicia Allaire, Mai X. Luong, and Kelly P. Smith
5  Reagent Preparation ......................................... 41
   Alicia Allaire, Mai X. Luong, and Kelly P. Smith
6  Preparation of Mouse Embryonic Fibroblasts for Culture of
   Human Embryonic Stem Cells .................................. 59
   Meng-Jiao Shi, Maria Borowski, and Kimberly Stencel
7  Thawing and Seeding of Frozen Mouse Embryonic Fibroblasts ... 87
   Meng-Jiao Shi, Maria Borowski, and Kimberly Stencel
8  Thawing, Seeding, and Changing the Medium of Human
   Embryonic Stem Cells ........................................ 95
   Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski
9  Passaging of Human Embryonic Stem Cells on Inactivated
   Mouse Embryonic Fibroblast Plates .......................... 105
   Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski
10 Harvesting Human Embryonic Stem Cells for
   Cryopreservation ........................................... 119
   Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski
11 Human Embryonic Stem Cell Culture on BD Matrigel™ with
   mTeSR®1 Medium ............................................. 129
   Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski

SECTION III  LABORATORY GUIDE FOR HUMAN STEM CELL
CULTURE: CHARACTERIZATION OF PLURIPOTENT STEM CELLS
12 Defining Pluripotency ...................................... 141
   Kelly P. Smith and Mai X. Luong
13 Characterization of Human Embryonic Stem Cells by
   Immunofluorescence Microscopy .............................. 149
   Shirwin M, Pockwinse and Prachi N. Ghule
14 Preparation of Human Embryonic Stem Cell Samples for Flow
   Cytometry .................................................. 159
   Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski
15 Characterization of Human Embryonic Stem Cell Gene
   Expression Using Reverse Transcription Polymerase Chain
   Reaction ................................................... 167
   Alicia Allaire, Kelly P. Smith, and Mai X. Luong
16 Determining Pluripotency of Human Embryonic Stem Cells:
   Embryoid Body Formation .................................... 191
   Shirwin M. Pockwinse and Prachi N. Ghule
17 Characterization of Human Embryonic Stem Cells by
   Cytogenetics: Karyotyping and Fluorescence In Situ
   Hybridization .............................................. 199
   Shirwin M. Pockwinse, Prachi N. Ghule and Anne Higgins
18 High-Resolution Chromatin Immunoprecipitation Assay ........ 211
   Beatriz Pérez-Cadahia, Bojan Drobic, and James R. Davie
19 Assaying Pluripotency Via Teratoma Formation ............... 217
   M. William Lensch and Tan A. Ince

SECTION IV  PERSPECTIVES IN HUMAN STEM CELL TECHNOLOGIES
20 Genomic Analysis of Pluripotent Stem Cells ................. 237
   David Lapointe
21 Proteomic Analysis of Human Pluripotent Cells .............. 245
   Andy T.Y. Lau, Yan-Ming Xu, and Jen-Fu Chiu
22 Bioinformatics Strategies for Understanding Gene
   Expression in Human Pluripotent Cells ...................... 257
   Gustavo Glusman, Bruz Marzolf, Kai Wang, Ji-Hoon Cho,
   Burak Kutlu, and Qiang Tian
23 Epigenetic Analysis of Pluripotent Cells ................... 273
   Mojgan Rastegar, Geneviève P. Delcuve, and James
   R. Davie
24 Differentiation of Pluripotent Stem Cells: An Overview ..... 289
   Jeremy Micah Crook
25 Cellular Reprogramming: Current Technology,
   Perspectives, and Generation of Induced Pluripotent
   Cells ...................................................... 297
   Tanja Dominko

SECTION V  APPLICATIONS OF HUMAN EMBRYONIC STEM CELLS
26 Human Pluripotent Cells: The Biology of Pluripotency ....... 313
   Li-Fang Chu and Thomas P. Zwaka
27 Human Pluripotent Cells for Regenerative Medicine:
   Potential Applications for Regenerative Medicine ........... 327
   Christopher C. Ford and Darreil N. Kotton
28 Therapeutic Applications of Human Embryonic Stem Cells ..... 339
   Shi-Jiang Lu, Irina Klimanskaya, Edmund Mickunas, and
   Robert Lanza
29 Human Stem Cells for Drug Screening, Toxicity,
   Specificity, and Off-Target Effects ........................ 353
   Arnaud Lacoste and Mark Burcin
30 Interfacing Stem Cells with Gene Therapy ................... 367
   Christian Mueller and Terence R. Flotte
31 Tissue Engineering for Stem Cell Mediated Regenerative
   Medicine ................................................... 377
   Janet Zoldan, Thomas P. Kraehenbuehl, Abigail K.R.
   Lytton-Jean, Robert S. Langer, and Daniel G. Anderson

Glossary ...................................................... 401
Index ......................................................... 413


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